Resumo Com o processo de globalização e as transformações no setor de telecomunicações, provenientes da reestruturação produtiva, os últimos 10 anos foram marcados pelo crescimento significativo do número de empresas terceirizadas na prestação de serviços de teleatendimento no Brasil. Este artigo objetivou descrever o processo metodológico da pesquisa-intervenção sobre perfil das condições de vida, de trabalho e de adoecimento dos teleoperadores do estado de Pernambuco. Esta é uma análise descritiva de pesquisa, ancorada na Abordagem Ecossistêmica em Saúde, centralizada na compreensão integrada da saúde humana, na construção do conhecimento direcionado para a ação. O projeto desenvolvido permitiu realizar um diagnóstico e iniciar o planejamento de intervenções a serem implementadas conjuntamente. A abordagem participativa permitiu a aplicação do diálogo sobre o conhecimento, que fortalece o desenvolvimento de ações na perspectiva da promoção da saúde, mas também de uma ciência cidadã baseada em pesquisa participativa.

Abstract In the wake of the globalization process and the transformations in the telecommunications sector, resulting from the productive restructuring, the last ten years in Brazil were marked by the significant increase in the number of telemarketing services being provided by outsourced companies. This article describes the methodological process of research-intervention on the profile of living, working and illness conditions of telemarketers in the state of Pernambuco. Its descriptive analysis is based on the Ecosystem Health, approach centered on the integrated understanding of human health for constructing action-oriented knowledge. The project developed allowed us to propose a diagnosis and start planning interventions to be implemented jointly. Its participatory approach favored a knowledge based on dialogue, practice that strengthens the development of health promoting actions and of a citizen science based on participatory research.

ABSTRACT Purpose: To evaluate the effect of hyperbaric oxygenation (HBO) on angiogenesis in random rat skin flaps, by immunoexpression of vascular endothelial growth factor A (VEGF-A). Methods: Forty adult rats were divided into four groups: GE) epilated; GE/HBO) epilated subjected to HBO; GER) epilated submitted to dorsal skin flap; GER/HBO) epilated subjected to dorsal skin flap + HBO. HBO was performed with rats inside a chamber under atmosphere close to 100% oxygen and pressure of 2.4 absolute atmospheres, 2h per day during seven consecutive days. GE and GER groups were placed in the hyperbaric chamber without HBO. Then, under anesthesia, skin flaps were removed and separated into three portions relative to pedicle fixation. The samples were fixed in formalin and processed for paraffin embedding. Histological sections were submitted to immunohistochemistry for VEGF-A detection. The number of immunostained-blood vessels were counted under light microscopy. Results: GE and GE/HBO groups showed normal and similar skin morphology in the three flap portions. A fibrin-leukocyte crust, along with denatured collagen and intense leukocyte infiltrate, was mainly observed in the dermis of the medial and distal flap portions of GER group. Meanwhile, the GER/HBO group presented more regions with intact collagen and small areas of leukocyte infiltrate in the three flap regions. VEGF-A-immunostained blood vessels were largely seen in all regions of GE and GE/HBO groups, whereas no significant differences were found between these groups. A decrease in vascularization was noticed in GER and GER/HBO groups, which was more evident in the most distal portion of the flaps. However, the number of VEGF-A-immunostained blood vessels in GER/HBO group was significantly higher when compared to GER group. Conclusions: Hyperbaric oxygenation was associated with increased angiogenesis and improved viability of rat skin flaps.

Resumo Com o processo de globalização e as transformações no setor de telecomunicações, provenientes da reestruturação produtiva, os últimos 10 anos foram marcados pelo crescimento significativo do número de empresas terceirizadas na prestação de serviços de teleatendimento no Brasil. Este artigo objetivou descrever o processo metodológico da pesquisa-intervenção sobre perfil das condições de vida, de trabalho e de adoecimento dos teleoperadores do estado de Pernambuco. Esta é uma análise descritiva de pesquisa, ancorada na Abordagem Ecossistêmica em Saúde, centralizada na compreensão integrada da saúde humana, na construção do conhecimento direcionado para a ação. O projeto desenvolvido permitiu realizar um diagnóstico e iniciar o planejamento de intervenções a serem implementadas conjuntamente. A abordagem participativa permitiu a aplicação do diálogo sobre o conhecimento, que fortalece o desenvolvimento de ações na perspectiva da promoção da saúde, mas também de uma ciência cidadã baseada em pesquisa participativa.

Abstract In the wake of the globalization process and the transformations in the telecommunications sector, resulting from the productive restructuring, the last ten years in Brazil were marked by the significant increase in the number of telemarketing services being provided by outsourced companies. This article describes the methodological process of research-intervention on the profile of living, working and illness conditions of telemarketers in the state of Pernambuco. Its descriptive analysis is based on the Ecosystem Health, approach centered on the integrated understanding of human health for constructing action-oriented knowledge. The project developed allowed us to propose a diagnosis and start planning interventions to be implemented jointly. Its participatory approach favored a knowledge based on dialogue, practice that strengthens the development of health promoting actions and of a citizen science based on participatory research.

Os óleos essenciais das folhas e caules de Rhodostemonodaphne parvifolia coletada na Reserva Florestal Adolpho Ducke foram obtidos por hidrodestilação e analisados por cromatografia em fase gasosa com detector de ionização de chama e espectrometria de massas. Treze constituintes foram identificados nas folhas, dos quais o ß-cariofileno foi o que apresentou maior percentual (41,30%). Nos caules foram identificados quinze compostos, com predominância dos sesquiterpenos ß-cariofileno (16,20%) e epi-cedrol (13,30%) e do monoterpeno linalol (15,40%). Os óleos foram analisados em ensaios químicos, de sequestro do radical livre DPPH, e enzimáticos, de inibição da enzima acetilcolinesterase. Nos ensaios de sequestro de radicais livres, apesar de terem se mostrado ativos em ensaios qualitativos, os óleos essenciais apresentaram fraca atividade antioxidante no ensaio quantitativo (inativos em concentração inferior a 1.000 µg/mL). A inibição da acetilcolinesterase só foi observada nos óleos essenciais das folhas. A composição química e a atividade biológica destes óleos estão sendo relatadas pela primeira vez neste trabalho.

The essential oils of leaves and stems of Rhodostemonodaphne parvifolia collected in the Adolpho Ducke Forest Reserve were obtained by hydrodistillation and analyzed by GC-FID and GC-MS. Thirteen components were identified in the leaf oil, of which ß-caryophyllene was the major (41.30%). Fifty components were identified in the stem oil, among them the sesquiterpenes ß-caryophyllene (16.20%) and epi-cedrol (13.30%), together with the monoterpene linalool (15.40%). The essential oils were analyzed at chemical, DPPH free radical scavenging; and enzymatic bioassays, acetylcholinesterase inhibition. At antioxidant DPPH assays, the oils showed to be active at the qualitative assay but only weakly active at quantitative test (inactive at concentration lower than 1,000 µg/mL). The inhibition of acetylcholinesterase was detected only in leaves essential oils. This paper describes for the first time the composition of these essential oils and their biological activities.